Pre-treatment method for clinical detection of respiratory virus samples
FANG Zhi-xin1 GU Hong-qin2 SHi Mei-fang3 LIN Yong
1.Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2.Department of Quality Control,Baoshan District Healthcare Service Center of Shanghai, Shanghai 201999, China;
3.Department of Laboratory,Baoshan District Healthcare Service Center of Shanghai, Shanghai 201999, China;
4.Department of Laboratory, Jing′an District Centre Hospital of Shanghai (Jing′an Branch of Huashan Hospital Affiliated to Fudan University), Shanghai 200040, China
Abstract：Respiratory virus infection is one of the main causes of common respiratory diseases.This article aims to summarize and discuss the elements of quality control of the pre-processing of respiratory virus samples, and to provide reference and guidance for clinical respiratory virus sample pre-processing programs.The main points of the quality control on the pretreatment of respiratory virus clinical testing are reviewed in this essay, including 4 main aspects-the type of specimen, collection, transportation and storage.According to the analysis, the applicable conditions of various types of specimens and swab materials, as well as the requirements for the collection, transportation and storage of respiratory virus samples, are obtained.Among them, nasopharyngeal swab specimens are the key specimen types.At present, in the selection of specimen types for respiratory virus samples, nasopharyngeal swab is the best sample type.Multiple types of specimens can also be used in combination.For example, nasopharyngeal swab and oropharyngeal swabs, saliva specimens, and sputum specimens can improve the sensitivity and accuracy of detection.To the actual clinical situation, it has certain value.In addition, this article also explains the possibility of new specimen types being put into clinical testing.
Howard F.Comparison of midturbinate flocked-swab specimens with nasopharyngeal aspirates for detection of respiratory viruses in children by the direct fluorescent antibody technique[J].J Clin Microbiol，2010，48（10）：3742-3743.
[5]
Kim C，Ahmed JA，Eidex RB，et al.Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-PCR assays[J].PLoS One，2017，6（6）：e21610.
[6]
Kim YG，Yun SG，Kim MY，et al.Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription-PCR[J].J Clin Microbiol，2016，55（1）：226-233.
[7]
Lambert SB，Whiley DM，O′Neill NT，et al.Comparing nosethroat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction[J].Pediatrics，2008，122（3）：e615-e620.
[8]
Li L，Chen QY，Li YY，et al.Comparison among nasopharyngeal swab，nasal wash，and oropharyngeal swab for respiratory virus detection in adults with acute pharyngitis[J].BMC Infect Dis，2013，13（1）：281.
Spencer S，Thompson MG，Flannery B，et al.Comparison of Respiratory Specimen Collection Methods for Detection of Influenza Virus Infection by Reverse Transcription-PCR：a Literature Review[J].J Clin Microbiol，2019，57（9）：e00027-19.
[12]
Hun JJ，Hee KK，Hwan JS，et al.Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses[J].J Med Virol，2014，86（12）：2122-2127.
[13]
Kw TK，Lu L，Cy YC，et al.Additional molecular testing of saliva specimens improves the detection of respiratory viruses[J].Emerg Microbes Infect，2017，6（6）：e49.
Falsey AR，Formica MA，Walsh EE.Yield of Sputum for Viral Detection by Reverse Transcriptase PCR in Adults Hospitalized with Respiratory Illness[J].J Clin Microbiol，2012，50（1）：21-24.
Catherine M，Sally C，Jaisi S，et al.Dry cotton or flocked respiratory swabs as a simple collection technique for the molecular detection of respiratory viruses using real-time NASBA[J].J Virol Methods，2008，153（2）：84-89.
[20]
Liesbeth VW，Hanne M，David DH，et al.Sampling variability between two mid-turbinate swabs of the same patient has implications for influenza viral load monitoring [J].Virol J，2014，11：105-113.
[21]
Wang X，Tan L，Wang X，et al.Comparison of nasopharyngeal and oropharyngeal swabs for SARS-CoV-2 detection in 353 patients received tests with both specimens simultaneously[J].Int J Infect Dis，2020，94：107-109.
[22]
Thompson MG，Ferber JR，Odouli R，et al.Results of a pilot study using self-collected mid-turbinate nasal swabs for detection of influenza virus infection among pregnant women[J].Influenza Other Respir Viruses，2015，9（3）：155-160.
[23]
Caselton Dl，Arunga G，Emukule G，et al.Does the length of specimen storage affect influenza testing results by real-time reverse transcription-polymerase chain reaction?an analysis of influenza surveillance specimens，2008 to 2010[J].Euro Surveill，2014，19（36）：20893.
[25]
Hoslkawa-Muto J，Fujinami Y，Mizuno N.Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics[J].J Forensic Leg Med，2015，34：29-33.
[26]
Miller JM，Binnicker MJ，Campbell S，et al.A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases：2018 Update by the Infectious Diseases Society of America and the American Society for Microbiology[J].Clin Infect Dis，2018，67（6）：e1-e94.
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