Abstract：Objective To explore the effect of Tifentai (TFT) on secretion of Hepatitis B surface antigen (HbsAg) in 2.2.15 cells.Methods Hepatitis B virus deoxyribonucleic acid (HBV-DNA) clones were used to transfect human hepatoma cells HepG2 2.2.15 cell line as experimental model,and TFT was prepared into 102,51,25.5 μm solution.Lamivdine (LAM) 218 μm positive control group (referred to as LAM 218 μm group) and virus control group were established,and they were added to 24-well cell culture plates,0.6 ml/well,4 wells per concentration.The solution was changed once every 4 days,and the cell supernatant was collected on the eighth day.The titer of HBsAg in the cell supernatant was determined by enzyme-linked immunosorbent assay (ELISA),and the OD values of the three batches in each group were compared,and the average inhibition rate was recorded.Results The OD value of the LAM 218 μm group was significantly lower than that of the virus control group,and the difference was statistically significant (P<0.05).The OD values of the TFT 102,51,and 25.5 μm group were significantly lower than those of the virus control group,and the differences were statistically significant (P<0.05).The average inhibition rates of the LAM 218 μm,TFT 102,51,and 25.5 μm group were 37.49%,56.26%,47.33%,and 36.82%,respectively.Conclusion TFT can effectively inhibit the secretion of HBsAg,thereby exerting its anti HBV effect,and can provide data support and reference for further research and development of TFT to become a new anti-HBV drug.
Meng FC，Xu WR，Li YZ，et al.In silico molecular docking study of repensine and bentysrepinine against HBV DNA polymerase[J].Chin Herb Med，2015，25（1）：39-44.
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